Automated Gating Concordance — Auto vs Manual Ground Truth

Validation of automated gating against expert manual gating across populations. Reports agreement (R², bias, Bland–Altman limits) and event-level overlap (F1) per population — and honestly flags where automation is reliable vs where a human still gates. Governed automation: use auto where it's validated, keep humans where it isn't.
Analysis demo by Kalif Shear
Flow Cytometry SME · CGT QC / Analytical

Automated vs Manual (%)

Each point = one sample. Dashed = identity (perfect agreement); solid = fitted line.

Bland–Altman Agreement

Difference (auto − manual) vs mean. Center = bias; dashed = 95% limits of agreement.

Concordance Summary — All Populations

Validation verdict per population. F1 = event-level gate-membership overlap between automated and manual gates.