% of parent at each gate
Gated on live CD3⁺ lymphocytes
CAR⁺ of CD3⁺ = product-defining frequency
Normalized full-spectrum emission across the detector array. In spectral cytometry, each fluorophore is identified by its whole signature — not a single detector. Click a fluorophore to isolate it.
Cosine similarity between signatures. High similarity (hot cells) = fluorophores that are hard to unmix and will spread spillover — the key panel-design risk to catch before running samples.
Single-stain reference vs unmixed output for the selected pair — clean separation vs residual spread.